Distribution and mating type characterization of chickpea blight (Didymella rabiei (Kov.) v. Arx) in Ethiopia
نویسندگان
چکیده
We have assessed the distribution and mating type of chickpea blight (Didymella rabiei (Kov.) v. Arx) from a total 350 272 fields in major (Cicer arietinum L.) growing areas Ethiopia for two consecutive cropping seasons 2017 2018, respectively. The prevalence D. was 20 % year 7% 2018. Severity range 2?9 incidence 10–100 were recorded both only 7–20 assed fields. Field 2018 significantly different (df = 31; p < 0.001 df 0.001, respectively). Similarly, disease 0.001). ratio frequency highly deviated (1:1) (X2 213.62 p= <0.001; X2 144.36 0.01). Genomic DNA Representative samples extracted single spore culture Mating 1 (MAT1-1) specific primer SP21, 2 (MAT1-2) Tail 5, flanking region-specific Com1 assay multiplexed PCR reaction to determine occurrence Ethiopia. Out 156 samples, 15 positive MAT1-1 (?10 %) with 9:1. MAT 1?2 most dominant possibly asexual reproduction is result important Ascochyta management breeding strategy.
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ژورنال
عنوان ژورنال: Current Plant Biology
سال: 2021
ISSN: ['2214-6628']
DOI: https://doi.org/10.1016/j.cpb.2021.100220